Multi-drug resistant ESKAPE pathogens and the uses of plants as their antimicrobial agents.

Infections by ESKAPE (Enterococcus sp., Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) pathogens cause major concern due to their multi-drug resistance (MDR). The ESKAPE pathogens are frequently linked to greater mortality, diseases, and economic burden in healthcare worldwide. Therefore, the use of plants as a natural source of antimicrobial agents provide a solution as they are easily available and safe to use. These natural drugs can also be enhanced by incorporating silver nanoparticles and combining them with existing antibiotics. By focussing the attention on the ESKAPE organisms, the MDR issue can be addressed much better.

Dataset of ultrasound-assisted extraction of anthocyanin from the petals of Clitoria ternatea using Taguchi method and effect of storage conditions on the anthocyanin stability.

Anthocyanins are natural water-soluble colourants with a number of reported health benefits and are an excellent alternative to artificial dyes. Anthocyanins from Clitoria ternatea were obtained using ultrasound-assisted extraction with glycerol-water (60:40 w/v). The anthocyanins in the extract were measured at wavelength 550 and 700 nm using a UV-visible spectrophotometer and expressed in terms of total anthocyanins content (TAC). Taguchi method was employed to optimize the extraction parameters that include the extraction time (30 to 50 min), extraction temperature (40 to 60 °C), and solvent to solid ratio (10:1 to 30:1), with TAC as the response. The obtained data showed the optimum extraction parameters as 30 min extraction time, 50 °C extraction temperature, and 10:1 solvent to solid ratio. The anthocyanin's storage stability was analyzed for 7 days at varying storage temperatures and exposure to light. The present dataset in this article indicated the glycerol-water system as a green alternative for anthocyanin extraction and acted as a storage medium. Furthermore, our methodology can be applied to optimize the anthocyanins extraction process, manipulate storage conditions and improve the extract quality.

Comparative Studies on Different Extraction Methods of Centella asiatica and Extracts Bioactive Compounds Effects on Antimicrobial Activities.

The interest of consumers in using products containing phytochemicals derived from plants is growing day by day due to the shift of consumers' preferences from convenience to environmental sustainability. One plant utilized in many products is Centella asiatica, a herb commonly used in folk medicine, health supplements, and beauty products. Extraction of bioactive compounds from C. asiatica was performed using conventional methods and modern methods (e.g., microwave or ultrasound-assisted and subcritical water extraction). This review summarizes the variety of methods used to extract active compounds from C. asiatica, their influence on the bioactive compounds and antimicrobial activity in vitro and in vivo, and the safety and toxicology of C. asiatica extract.

Biomedical Applications of Bacterial Exopolysaccharides: A Review.

Bacterial exopolysaccharides (EPSs) are an essential group of compounds secreted by bacteria. These versatile EPSs are utilized individually or in combination with different materials for a broad range of biomedical field functions. The various applications can be explained by the vast number of derivatives with useful properties that can be controlled. This review offers insight on the current research trend of nine commonly used EPSs, their biosynthesis pathways, their characteristics, and the biomedical applications of these relevant bioproducts.